Lyn- and ERK-mediated vs. Ca2+ -mediated neutrophil O responses with thermal injury.
نویسندگان
چکیده
We evaluated the dependency of neutrophil O production on PTK-Lyn and MAPK-ERK1/2 in rats after thermal injury. Activation of PTK-Lyn was assessed by immunoprecipitation. Phosphorylation of ERK1/2 was assessed by Western blot analysis. O production was measured by isoluminol-enhanced luminometry. Imaging technique was employed to measure neutrophil [Ca2+](i) in individual cells. Thermal injury caused marked upregulation of Lyn and ERK1/2 accompanying enhanced neutrophil O production. Treatment of rats with PTK blocker (AG556) or MAPK blocker (AG1478) before burn injury caused complete inhibition of the respective kinase activation. Both AG556 and AG1478 produced an ~66% inhibition in O production. Treatment with diltiazem (DZ) produced an ~37% inhibition of O production without affecting Lyn or ERK1/2 activation with burn injury. Ca2+ mobilization was upregulated with burn injury but not affected by treatment of burn rats with AG556. Unlike the partial inhibition of burn-induced O production by AG556, AG1478, or DZ, platelet-activating factor antagonist (PAFa) treatment of burn rats produced near complete inhibition of O production. PAFa treatment also blocked activation of Lyn. The findings suggest that the near complete inhibition of O production by PAFa was a result of blockade of PTK as well as Ca2+ signaling. Overall, our studies show that enhanced neutrophil O production after thermal injury is a result of potentiation of Ca2+ -linked and -independent signaling triggered by inflammatory agents such as PAF.
منابع مشابه
Lyn- and ERK-mediated vs. Ca -mediated neutrophil O2 responses with thermal injury
Fazal, Nadeem, Walid M. Al-Ghoul, Megan J. Schmidt, Mashkoor A. Choudhry, and Mohammed M. Sayeed. Lynand ERK-mediated vs. Ca2 -mediated neutrophil O2 responses with thermal injury. Am J Physiol Cell Physiol 283: C1469–C1479, 2002. First published June 20, 2002; 10.1152/ajpcell.00114.2002.—We evaluated the dependency of neutrophil O2 production on PTK-Lyn and MAPKERK1/2 in rats after thermal inj...
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ورودعنوان ژورنال:
- American journal of physiology. Cell physiology
دوره 283 5 شماره
صفحات -
تاریخ انتشار 2002